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为探讨中国恶性肿瘤高发区粮食中优势污染霉菌毒素—杂色曲霉素 (Sterigmatocystin,ST)的致癌作用 ,运用银染 PCR- SSCP方法分析了不同浓度的 ST(1μg/ ml和 3μg/ m l)对体外培养的人胚肺细胞恶性转化过程中抑癌基因 p5 3第 5、6、7、8外显子及癌基因 Ki- ras的突变情况。结果显示 ST处理后第 2 2周 ,人胚肺成纤维细胞 p5 3基因的第 8外显子和 Ki- ras癌基因均出现异常泳动带型 ,表明 ST诱发了抑癌基因 p5 3及癌基因 Ki- ras突变 ,进一步证实了 ST对人肺组织的致癌作用。
In order to investigate the carcinogenic effect of mycotoxin Sterigmatocystin (ST), a predominant contaminant of grain in the high-risk areas of malignant tumors in China, different concentrations of ST (1 μg/ml and 3 μg/ml) were analyzed by silver staining PCR-SSCP method. The mutations of exon 5, 6, 7, and 8 of tumor suppressor gene p53, and oncogene Kiras in the malignant transformation of cultured human embryonic lung cells in vitro. The results showed that at the 22nd week after ST treatment, the exon 8 and Ki-ras oncogene of p53 gene in human embryonic lung fibroblasts showed abnormal mitotic pattern, indicating that ST induced the tumor suppressor gene p53 and cancer. Gene mutations of Ki-ras further confirmed the carcinogenic effects of ST on human lung tissue.