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[目的]探究丙肝肝硬化发展为肝癌的分子机制,为肝癌的早期诊断和治疗提供新思路。[方法]利用生物信息学手段,根据肝硬化和肝癌两种状态下基因间的相关系数变化寻找差异共表达基因,同时筛选甲基化基因,整合miRNA—靶点、转录因子—靶点的调控关系,构建miRNA—转录因子—基因的调控网络并进行拓扑学分析。[结果]确定了肝硬化和肝癌中包含miR-590-3p、TSG101、EGR1、MME在内的主要hub因子。与肝硬化相比,肝癌中的基因间相关系数总体呈下降趋势,hub因子相关的基因间也以相关系数降低为主。[结论]miRNA—转录因子—基因的调控网络可以系统地分析肝癌的分子机制,网络中的hub因子可以作为潜在的肿瘤标志物用于肝癌的早期诊断和治疗。
[Objective] To explore the molecular mechanism of hepatitis C cirrhosis into hepatocellular carcinoma and to provide new ideas for the early diagnosis and treatment of liver cancer. [Methods] The co-expression genes were searched for by bioinformatics methods based on the correlation coefficient between genes in cirrhosis and hepatocellular carcinoma, and the methylation genes were screened for integration of miRNA-target and transcription factor-target regulation Relationship, construct miRNA-transcription factor-gene regulatory network and topology analysis. [Results] The main hub factors including miR-590-3p, TSG101, EGR1 and MME in cirrhosis and liver cancer were identified. Compared with cirrhosis, the correlation coefficients of genes in hepatocellular carcinoma generally showed a decreasing trend, and the correlation coefficients of genes related to hub factors also decreased. [Conclusion] The miRNA-transcription factor-gene regulatory network can systematically analyze the molecular mechanism of liver cancer. The hub factor in the network can be used as a potential tumor marker for the early diagnosis and treatment of liver cancer.