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目的观察补肾填精法对糖尿病勃起功能障碍(ED)大鼠性功能水平的影响及作用机制。方法采用SD大鼠腹腔注射链脲佐菌素法,造模后断尾取血检测血糖,当血糖大于16.6 mmol/L并颈项皮肤注射阿朴吗啡后无勃起,提示糖尿病勃起功能障碍大鼠模型造模成功。将造模成功的大鼠随机分为补肾填精法治疗组、阳性对照组和模型组各10只,同时留10只大鼠作为空白对照组,各组在给药8周时和停药2周后分别测定大鼠的性功能水平,同时检测大鼠阴茎组织形态学及一氧化氮合成酶(NOS)。结果补肾填精法治疗组大鼠阴茎组织平滑肌数量较模型组及阳性对照组有所增多,同时可使纤维密度降低及血管壁变薄。补肾填精法治疗组和阳性对照组NOS表达量分别为(64.1±5.91)和(34.0±4.81),与模型组(22.8±4.47)比较均有所提高,差异具统计学意义(P<0.01)。与空白对照组相比,模型组大鼠性功能显著下降,差异具统计学意义(P<0.01);补肾填精法治疗组和阳性对照组均可改善大鼠性功能,与模型组比较差异具统计学意义(P<0.01),补肾填精法治疗组远期疗效优于阳性对照组,差异具统计学意义(P<0.05)。结论补肾填精法能显著提高糖尿病勃起功能障碍大鼠的性功能并维持一定的时间,这可能与其能改善阴茎组织形态学及提高NOS表达水平有关。
Objective To observe the effects and mechanism of Bushen Filling and Semen Decoction on the sexual function of diabetic rats with erectile dysfunction (ED). Methods Sprague Dawley rats were injected intraperitoneally with streptozotocin (STZ). Blood samples were taken from the tail of the SD rats for blood glucose measurement. When the blood glucose was more than 16.6 mmol / L and no apomorphine was injected into the skin of the neck, the diabetic model of erectile dysfunction Modeling success. The successful model rats were randomly divided into Bushenjingjing group, positive control group and model group, each with 10 rats while 10 rats were taken as blank control group. Weeks later, the sexual function of the rats was measured, and the penis histomorphology and nitric oxide synthase (NOS) were detected. Results Compared with the model group and the positive control group, the number of smooth muscle in the penis of rats treated with Bushenzhijing Decoction increased, while the fiber density decreased and the blood vessel wall became thinner. The expression of NOS in Bushenjingjing group and positive control group were (64.1 ± 5.91) and (34.0 ± 4.81) respectively, which were significantly higher than those in model group (22.8 ± 4.47) (P <0.01) ). Compared with the blank control group, the sexual function of the model group was significantly decreased, the difference was statistically significant (P <0.01); The Kidney and Seminal Filtration treatment group and the positive control group can improve the sexual function of rats, compared with the model group With statistical significance (P <0.01), the long-term curative effect of Bushenjingjing group was better than that of positive control group (P <0.05). Conclusion Bushenzhengjing method can significantly improve the sexual function of diabetic erectile dysfunction rats and maintain a certain time, which may be related to its ability to improve penile morphology and improve the expression of NOS level.