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目的:探讨维生素D受体(VDR)基因BsmⅠ酶切位点多态性与深圳市成人迟发性自身免疫性糖尿病(LADA)的关系。方法:对25例LADA、32例2型糖尿病(T2DM)患者和30例正常人(NC)采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析VDR-BsmⅠ位点基因型。结果:T_2DM患者VDR基因B等位基因频率明显高于正常对照组(20.3%vS.6.7%,P<0.05),基因型Bb明显高于正常对照组(40.6%vS.13.3%,P<0.05),基因型bb明显低于正常对照组(59.4%vS.86.7%,P<0.05);LADA患者Bb基因型频率以及B等位基因频率与对照组比较差异无统计学意义(P>0.05)。结论:VDR基因BsmⅠ位点多态性与LADA易感性可能无关,但与T_2DM的易感性密切相关。
Objective: To investigate the relationship between polymorphism of Bsm Ⅰ site of vitamin D receptor (VDR) gene and adult delayed autoimmune diabetes (LADA) in Shenzhen. Methods: The gene of VDR-BsmⅠ gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 25 cases of LADA, 32 cases of type 2 diabetes mellitus (T2DM) and 30 normal subjects (NC) type. Results: The B allele frequency of VDR gene in T 2 DM patients was significantly higher than that in control subjects (20.3% vs 7.6%, P <0.05), and genotype Bb was significantly higher than that in controls (40.6% vs 13.3%, P 0.05 ), Genotype bb was significantly lower than that of the normal control group (59.4% vS. 86.7%, P <0.05). There was no significant difference in Bb genotype frequencies and B allele frequencies between control group and LADA patients (P> 0.05) . Conclusion: The Bsm Ⅰ polymorphism of VDR gene may not be related to LADA susceptibility, but it is closely related to the susceptibility of T 2 DM.