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用带有ｇｕｓ报告基因的农杆菌ＬＢＡ４４０４／ｐＣＡＭＢＩＡ１３０１ 转化水稻品种鄂宜１０５ 成熟胚诱导的愈伤组织，以ｇｕｓ基因瞬间表达活性为指标测定转化频率，对于利用ｐＣＡＭＢＩＡ 表达载体系列进行水稻遗传转化的最佳条件进行了初步的研究．结果表明：最适菌液浓度值为Ｄ（６００）＝ １．０；最适共培养温度为２２～２８ ℃；共培养培养基的ｐＨ 值在５．２～６．２ 这一较宽的范围内均可．共培养时间以不超过３ ｄ 为宜；在共培养培养基中加入乙酰丁香酮对提高转化效率非常重要，而在菌液中加入乙酰丁香酮处理对提高转化效率作用不大．但不同品种，甚至不同转化受体之间存在一定的差别，其最佳转化条件应视具体情况在此基础上加以摸索．
Agrobacterium tumefaciens LBA4404 / pCAMBIA1301 with gus reporter gene was used to transform callus induced from mature rice embryo of Ery 105, and the transient expression of gus gene was used as an indicator to determine the transformation frequency. For the genetic transformation of rice using pCAMBIA expression vector Good conditions for a preliminary study. The results showed that the optimum concentration of bacterial liquid was D (600) = 1.0. The optimum co-culture temperature was 22-28 ℃. The co-culture medium had a pH value of 5.2-6.2 Range can be. Co-cultivation time is less than 3 days is appropriate; adding acetosyringone to the culture medium is very important to improve the transformation efficiency, while the addition of acetosyringone in the bacteria liquid has little effect on improving the transformation efficiency. However, there are some differences between different varieties and even different transformation receptors. The optimal transformation conditions should be explored on this basis according to the specific conditions.