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To study the anticlastogenic effect of redistilled cow’s urine distillate (RCUD) in human peripheral lymphocytes (HLC) challenged with manganese dioxide and hexavalent chromium. Methods The anticlastogenic activity of redistilled cow’s urine distillate was studied in human polymorphonuclear leukocytes (HPNLs) and human peripheral lymphocytes in vitro challenged with manganese dioxide and hexavalent chromium as established genotoxicants and clastogens which could cause induction of DNA strand break, chromosomal aberration and micronucleus. Three different levels of RCUD: 1μL/mL, 50μL/mL and 100μL/mL, were used in the study. Results Manganese dioxide and hexavalent chromium caused statistically significant DNA strand break, chromosomal aberration and micronucleus formation, which could be protected by redistilled cow’s urine distillate. Conclusion The redistilled cow’s urine distillate posseses strong antigenotoxic and anticlastogenic properties against HPNLs and HLC treated with Cr+6 and MnO2. This property is mainly due to the antioxidants present in RCUD.
To study the anticlastogenic effect of redistilled cow’s urine distillate was studied in human polymorphonuclear leukocytes (HPNLs) and human peripheral lymphocytes (RCLD) challenged with manganese dioxide and hexavalent chromium. in vitro challenged with manganese dioxide and hexavalent chromium as established genotoxicants and clastogens which could cause induction of DNA strand break, chromosomal aberration and micronucleus. Three different levels of RCUD: 1 μL / mL, 50 μL / mL and 100 μL / mL, were used in the study. Results Manganese dioxide and hexavalent chromium caused fragments of significant DNA strand break, chromosomal aberration and micronucleus formation, which could be protected by redistilled cow’s urine distillate. Conclusion The redistilled cow’s urine distillate posseses strong antigen and anticlastogenic properties against HPNLs and HLC treated with Cr +6 and MnO2. This property is mainly due to the antioxidants present in RCUD.