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近20年来在小鼠、大鼠等啮齿类动物的颌下腺中陆续发现并分离出20多种生物活性多肽,如神经生长因子、肾素、血管紧张素、淋巴样因子等,其含量以雄性小鼠最为丰富,主要定位于颌下腺的颗粒曲符细胞(简称GCT)内。有些研究工作提示颌下腺有内分泌样功能,可能属于弥散性神经内分泌系统的一部分,但至今尚未定论。鉴于神经元特异性烯醇酶(简称NSE)是神经元和神经内分泌系统的特异性标记物,也是这些细胞分化成熟的标记物之一,应用免疫组织化学方法,观察NSE在颌下腺内的分布与细胞内定位,无疑可为进一步弄清颌下腺的生理作用提供必要的形态学基础。 方法 成年SD大鼠各3～4只,分别进行光镜与电镜免疫组织化学(组化)的观察。光镜观察组制成厚度为7μm的石蜡切片,以兔抗牛和抗大鼠NSE血清(抗NSEb和抗NSEr)-PAP免疫组化染色,抗血清稀释分别为1:200和1:400。电镜观察组的动物先经4℃ 4％多聚甲醛-0.2％戊二醛混合液心脏
Nearly 20 years in mice, rats and other rodents in the submandibular gland successively found and isolated more than 20 kinds of bioactive peptides, such as nerve growth factor, renin, angiotensin, lymphoid factor, the content of male small The most abundant mouse, mainly located in the submandibular glandular granule cells (referred to as GCT). Some studies suggest that the submandibular gland endocrine-like function may be part of the diffuse neuroendocrine system, but so far not yet conclusive. Since neuron-specific enolase (NSE) is a specific marker of neurons and neuroendocrine system, it is also one of the markers of differentiation and maturation of these cells. Immunohistochemistry was used to observe the distribution of NSE in the submandibular gland Intracellular localization can undoubtedly provide the necessary morphological basis for further understanding of the physiological role of the submandibular gland. Methods Three to four adult Sprague-Dawley rats were used for immunohistochemistry (light microscope and electron microscope) respectively. The light microscope observation group made the paraffin section with the thickness of 7μm. The anti-serum was diluted to 1: 200 and 1: 400 respectively by rabbit anti-cattle and anti-rat NSE serum (anti-NSEb and anti-NSEr) -PAP immunohistochemical staining. Animals in the electron microscope observation group were subjected to 4% paraformaldehyde-0.2% glutaraldehyde mixture heart at 4 ° C