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采用超高效液相色谱-四级杆飞行时间高分辨质谱联用技术(UHPLC-QTOF/MS)对RA264.7巨噬细胞甘油磷脂成分进行结构推断,运用修饰后的Bligh-Dyer法提取甘油磷脂,以流动相A:水(含10 mmol·L~(-1)乙酸铵和0.25%乙酸),流动相B:乙腈-异丙醇(1∶1)(含10 mmol·L~(-1)乙酸铵和0.25%乙酸)进行梯度洗脱,在ESI正负离子模式下进行数据采集。本实验推断出82个甘油磷脂成分,其中包括57个磷脂酰胆碱(PCs)、21个磷脂酰乙醇胺(PEs)、3个磷脂酰甘油(PGs)和1个磷脂酰肌醇(PI)。本文建立的基于UHPLC-QTOF/MS液质联用技术的RAW264.7巨噬细胞甘油磷脂类成分分析方法快速、简便、可靠。
The structures of glycerophospholipids in RA264.7 macrophages were elucidated by UHPLC-QTOF / MS and the glycerophospholipids were extracted by modified Bligh-Dyer method (Mobile phase A: water containing 10 mmol·L -1 ammonium acetate and 0.25% acetic acid), mobile phase B: acetonitrile-isopropanol (1: 1) containing 10 mmol·L -1 ) Ammonium acetate and 0.25% acetic acid) gradient elution in ESI positive and negative ion mode data acquisition. This experiment deduced 82 glycerophospholipid components, including 57 phosphatidylcholines (PCs), 21 phosphatidylethanolamines (PEs), 3 phosphatidylglycerols (PGs) and 1 phosphatidylinositol (PI). The established method of component analysis of RAW264.7 macrophage glycerophospholipids based on UHPLC-QTOF / MS LC / MS is rapid, simple and reliable.