对105例产前诊断对象作了羊水成活细胞和成活率分析,并采用改良的羊水细胞培养技术,进行染色体病产前诊断。通过改进羊水换液方法,建立羊水细胞收获标准,改进收获羊水细胞技术,选择和合并采用各种染色体显带技术,提高了羊水细胞培养成功率(99.05％),缩短了培养时间(平均7.6天)。4例严重血性羊水细胞培养(每毫升羊水红细胞数超过7.7×10~7)获得成功。对11个特殊高危家系实施了预防性优生取得成功。 105 cases of prenatal diagnosis of amniotic fluid surviving cells and survival rate analysis, and improved amniotic fluid cell culture technology, prenatal diagnosis of chromosomal disease. By improving amniotic fluid exchange method, establishing amniotic fluid cell harvesting standard, improving amniotic fluid cell harvesting technique, selecting and using various chromosome banding techniques, the success rate of amniotic fluid cell culture (99.05%) was improved and the culture time was shortened (7.6 days on average ). Four cases of severe bloody amniotic fluid cell culture (per ml of amniotic fluid more than 7.7 × 10 7) was successful. Preventive eugenics achieved success in 11 special high-risk pedigrees.