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目的研究腹腔注射不同剂量角叉菜胶建立贴近临床研究的动物血栓模型。方法按照体重将大鼠随机分为6组:空白组,5个剂量(10,15,20,25和30mg·kg~(-1))模型组(分别为模型A、B、C、D、E组),每组7只。空白组注射0.9%Na Cl 2 m L。测量6,12,18和24 h大鼠血栓黑尾长度,在24 h对大鼠尾部静脉采血,涂片观察血细胞形态,同时应用解剖病理学观察腹腔肠系膜病理学变化。结果在环境温度(18±2)℃、6 h时,血栓诱导率达100%。与模型A组和模型E组相比,模型C组的血栓黑尾长度在各时间点差异均有统计学意义(P<0.05);模型C组在24 h时血栓黑尾比率为80.1%。模型C组的大鼠肠系膜无病变产生,腹腔内无积水,同时血细胞发生较为明显的凝集并形成血栓。结论 20 mg·kg~(-1)角叉菜胶腹腔注射可以建立安全和贴近临床研究的动物病理血栓模型。
Objective To study the establishment of animal thrombosis model close to clinical study by intraperitoneal injection of different doses of carrageenan. Methods According to body weight, rats were randomly divided into 6 groups: blank group, 5 doses of 10, 15, 20, 25 and 30 mg · kg -1 model groups (model A, B, C, D, Group E), 7 in each group. The blank group was injected with 0.9% Na Cl 2 m L. The length of black tail of thrombus was measured at 6, 12, 18 and 24 h. Blood was collected from tail vein of rats at 24 h. The morphology of blood cells was observed by smear. At the same time, the pathological changes of mesenteric mesentery were observed by anatomical pathology. Results Thrombus induction rate was 100% at 6 h at ambient temperature (18 ± 2) ℃. Compared with model group A and model group E, the length of black tail of model group C was significantly different at all time points (P <0.05). The rate of thrombus black tail in group C was 80.1% at 24 h. Rats in model group C showed no pathological changes of mesentery, no intraperitoneal water, and more obvious blood coagulation and thrombus formation. Conclusions Intraperitoneal injection of 20 mg · kg -1 carrageenan can establish animal model of pathological thrombosis in safety and close clinical study.