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用放射免疫法检测支气管上皮细胞（ＢＥＣ）和肺泡巨噬细胞（ＡＭ）血管活性肠肽（ＶＩＰ）的分泌，放射配基法测定ＢＥＣ的ＶＩＰ受体（ＶＩＰＲ）表达，观察表皮生长因子（ＥＧＦ）的调控效应。结果示：①电镜下，ＢＥＣ细胞内有神经肽分泌颗粒特征，ＢＥＣ及ＡＭ均有ＶＩＰ基础分泌；②ＥＧＦ呈剂量依赖性促进ＢＥＣ的ＶＩＰ分泌，并促进ＡＭ分泌ＶＩＰ；③ＥＧＦ呈剂量依赖性上调ＢＥＣ的ＶＩＰＲ表达。提示气道上皮存在ＶＩＰ的自分泌或旁分泌，其保护效应受局部生长因子的调控。
The secretion of vasoactive intestinal peptide (VIP) in bronchial epithelial cells (BEC) and alveolar macrophages (AM) was detected by radioimmunoassay. The VIP receptor (VIPR) expression in BEC was detected by radioligand assay. The expressions of epidermal growth factor ) Regulatory effect. The results showed that: ① electron microscopy, BEC cells within the neuropeptide secreted particles, BEC and AM have VIP basic secretion; ② EGF in a dose-dependent manner to promote the secretion of BEC VIP, and promote the secretion of AM VIP; ③ EGF dose-dependently increased BEC VIPR expression. Tip of the airway epithelium exists VIP autocrine or paracrine, the protective effect of local growth factor regulation.