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通过单粒传法构建了含130个家系的AR z×扬麦158 F6∶7重组自交系群体(R IL),并采用田间病圃自然发病和喷洒悬浮孢子液两种方法对该群体进行赤霉病田间抗性鉴定;利用SSR标记对群体中控制抗赤霉病性的QTL进行定位分析。结果表明:群体中各家系的病情指数在所有试验中都存在很大的变异,在2002年、2003年和2005年其变异幅度分别为20.0%~80.0%,10.6%~74.6%和33.2%~89.0%;不同年份间的病情指数具有中等程度的相关性,且都达到了极显著水平(P<0.000 5);Xgwm114、Xgwm296、Xgwm111.2等15个SSR标记位点与群体抗赤霉病性显著相关,这些位点分别位于2DL、3BL和7DL等染色体上;经区间作图分析发现位于染色体7D上的Xwm c 94~Xwm c273.2区间存在一个抗赤霉病QTL,它在3年试验中的LOD值分别为1.5、2.6和2.0,可解释病情指数变异率的5.8%、8.7%和6.7%,Xgwm114是与该抗赤霉病QTL紧密连锁的标记位点,位于该抗性QTL的峰值区域。
The population of AR z × Yangmai 158 F6: 7 recombinant inbred lines (RIL) with 130 pedigrees was constructed by single grain propagation method. The population was subjected to natural disease in the field nursery and spores suspension spraying Sclerotinia sclerotiorum and Fusarium graminearum. The QTLs controlling the resistance to scab in the population were analyzed by SSR markers. The results showed that there was a great variation in the disease index of all families in all groups, with the variation ranged from 20.0% to 80.0%, from 10.6% to 74.6% and from 33.2% in 2002, 2003 and 2005, respectively ~ 89.0%. The disease index in different years had a moderate degree of correlation, and both reached extremely significant levels (P <0.0005). Fifteen SSR markers such as Xgwm114, Xgwm296 and Xgwm111.2 were not associated with the resistance These loci were located on chromosomes 2DL, 3BL and 7DL, respectively. According to the interval mapping analysis, there was a QTL for resistance to Fusarium head blight in Xwm c 94 ~ Xwm c273.2 located on chromosome 7D, The annual LOD values were 1.5, 2.6 and 2.0, respectively, which explained 5.8%, 8.7% and 6.7% of the variability of disease index. Xgwm114 was a marker locus closely linked to the QTL for Fusarium head blight resistance Peak area of QTL.