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目的:用高效液相色谱法测定颈舒胶囊中葛根素的含量,以确定制剂的定量标准。方法:以NH柱合硅胶为填充剂,以乙腈、水(40∶60)为流动相,检测波长为250nm。结果:葛根素在5.2~104.0μg/mL范围与其峰面积积分值之间线性关系良好,平均回收率97.6%,葛根素含量为0.045%。结论:高效液相色谱法可测定颈舒胶囊中葛根素的含量,明确葛根素的定量标准。
Objective: To determine the content of puerarin in Jingshu capsule by HPLC, and to determine the quantitative standard of preparation. Methods: The column was packed with NH silica gel as mobile phase and acetonitrile and water (40:60) as mobile phase. The detection wavelength was 250 nm. Results: There was a good linear relationship between the concentration of puerarin and the integral value of peak area in the range of 5.2-104.0 μg / mL with an average recovery of 97.6% and puerarin content of 0.045%. Conclusion: The HPLC method can be used to determine the content of puerarin in Jingshu capsule and to confirm the quantitative standard of puerarin.