通过结扎大鼠一侧大脑中动脉,同时夹闭双侧颈总动脉30min或60min后再通,制成局灶性皮质脑梗死再灌流动物模型,行为学观察可见各种神经缺损体征。病理,电镜观察发现梗死灶位于一侧大脑皮质。结果提示该方法成功率高、重复性好、动物存活时间长,是研究脑再灌流损伤较理想的动物模型。 Through the ligation of the rat middle cerebral artery, bilateral occlusion of the common carotid artery at the same time 30min or 60min after the pass, made of focal cortical cerebral infarction reperfusion animal model, a variety of signs of neurological deficits can be seen. Pathology, electron microscopy found that infarcts located in one side of the cerebral cortex. The results suggest that this method has high success rate, good repeatability, and long survival time of animals. It is an ideal animal model for studying brain reperfusion injury.