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通过构建快生型大豆根瘤菌Ｂ５２的基因文库和三亲本杂交，将增效因子ＤＮＡ片段导入优良的慢生型大豆根瘤菌２２－１０中，获得携带来自快生菌增效因子ＤＮＡ片段的工程菌株ＨＮ３２，经盆栽和小区试验，证明基因工程菌株ＨＮ３２比出发菌株２２－１０平均增产６％，比对照平均增产１３．２％～１６．９％，相当于每公顷施７５～１５０ｋｇ尿素．１９９２～１９９５年，在广西推广应用基因工程大豆根瘤菌ＨＮ３２２．１６万ｈｍ２，每公顷平均增产１９％，投入产出比１：３０。增加经济效益１４０９．８万元。
By constructing the gene library of fast-growing soybean rhizobia strain B52 and the three-parental hybridization, the efficiency factor DNA fragment was introduced into an excellent slow-growth soybean rhizobium strain 22-10 to obtain a project carrying the DNA fragment from an accelerated bacteria synergistic factor The strain HN32 showed that 6% of the genetic engineering strain HN32 had an average increase of 6% over the original strain 22-10 and 13.2% ~ 16.9% of the average increase of the control strain, which was equivalent to 75-150 kg of urea per hectare. From 1992 to 1995, the genetic engineering soybean Rhizobium strain HN3221600 hm2 was popularized and applied in Guangxi, with an average increase of 19% per hectare and an input-output ratio of 1:30. Increase the economic benefits of 14098000 yuan.