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目的探讨P504S、P63、ck/34BE12三项指标联合检测对前列腺良恶性病变的病理鉴别诊断的意义。方法收集40例前列腺良恶性病理活检及切除标本进行常规HE及免疫组织化学观察并结合文献讨论。结果正常前列腺基底细胞染色ck/34BE12(98%)、P63(100%)、P504S(-);前列腺癌染色P63(-)、P504S(+)、ck/34BE12(-);PIN染色P63(+)、P504S(+)、ck/34BE12(+);良性病变P63(+)、P504S(-)、ck/34BE12(+)。结论P504S染前列腺腺癌和PIN胞浆,P63、ck/34BE12染PIN和良性前列腺基底细胞核。在同一张切片上,同时用两种染色显示基底细胞和癌细胞,良性腺体为单一的P63、ck/34BE12着色,恶性腺体为单一的P504S着色,二者同时显色的腺体多半是PIN,因此,作为诊断前列腺癌的阴性抗体(P63、ck/34BE12)与阳性抗体(P504S)联合应用是鉴别前列腺肿瘤良恶性的最佳指标。
Objective To investigate the significance of the combined detection of P504S, P63 and ck / 34BE12 in differential diagnosis of benign and malignant prostatic diseases. Methods 40 cases of benign and malignant prostate biopsy and resection specimens were collected for routine HE and immunohistochemical observation combined with the literature. RESULTS: Prostate carcinoma stained P63 (-), P504S (+), ck / 34BE12 (-), P63 (+), P63 ), P504S (+), ck / 34BE12 (+); benign lesions P63 (+), P504S (-), ck / 34BE12 (+). Conclusions P504S stained for prostate adenocarcinoma and PIN cytoplasm, P63, ck / 34BE12 stained PIN and benign prostatic basal cell nucleus. On the same slice, basal cells and cancer cells were also shown by two stains. The benign glands were single P63 and ck / 34BE12 were stained. The malignant glands were single P504S stained. The color glands of both were mostly Therefore, the combination of negative antibody (P63, ck / 34BE12) and positive antibody (P504S) for the diagnosis of prostate cancer is the best indicator to identify benign and malignant prostate tumors.