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目的:建立研究同一地域来源、不同树龄的凹叶厚朴7种次生代谢产物质量分数变化,为确定凹叶厚朴最佳采收条件提供参考依据.方法:利用高效液相色谱法(HPLC)对绿原酸、芦丁、金丝桃苷、槲皮苷、槲皮素、和厚朴酚与厚朴酚进行分离,并建立标准曲线及回归方程.分析随着树龄的增加,其叶、枝皮、茎皮和根皮中7种次生代谢产物质量分数积累情况.结论:不同树龄的凹叶厚朴叶片、枝皮、茎皮和根皮中7种代谢产物质量分数随着树龄积累差异较大,绿原酸、芦丁、金丝桃苷、槲皮苷和槲皮素等主要存在于在树龄相对较小的叶片和枝皮中,随着树龄从7年增加至26年其积累变化不明显;厚朴酚与和厚朴酚主要分布于凹叶厚朴的树皮中,其中根皮质量分数最高,茎皮、枝皮次之;7至13年树龄的植株,其厚朴酚与和厚朴酚积累量随树龄增加而明显增加,13至26年树龄的植株随树龄增加二者积累量增幅不大.因此,凹叶厚朴的最适采收对象是树龄超过13年之后的植株.
OBJECTIVE: To establish a method to study the changes of seven secondary metabolites of Magnolia officinalis from different geographical origins and ages, and to provide a reference for determining the optimum harvesting conditions of Magnolia officinalis.Methods: Acid, rutin, hyperoside, quercetin, quercetin, honokiol and honokiol were separated, and the standard curve and regression equation were established.With the increase of tree age, the leaf, shoots, Stem bark and root bark of seven kinds of secondary metabolites.Conclusion: The mass fraction of seven metabolites in leaves, branches, stem bark and root bark of different ages of Euonymus japonicus var. The contents of acid, rutin, hyperoside, quercitrin and quercetin mainly existed in leaves and branches with relatively small age, but their accumulation did not change significantly with the increase of tree age from 7 to 26 years Magnolol and honokiol were mainly distributed in the bark of Magnolia officinalis, with the highest root bark quality, followed by the bark and branch bark. The plants with the age of 7 to 13 years accumulated magnolol and honokiol The amount increased significantly with age, 13 to 26 years old trees with trees Increase in both the amount of accumulation is not increased. Therefore, the optimal harvest Magnolia biloba is an object old plant more than 13 years later.