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利用双向电泳技术,对栽培小麦(AABBDD)、染色体代换系(6V/6A)、易位系(6VS/6AL)、(6VS/6DL)和簇毛麦(VV)的叶片全蛋白进行了比较研究。在栽培小麦、代换系和两个易位系中检测到超过350个蛋白组分,它们的分子量范围是10-110 KD,等电点在4.5-8.6之间。栽培小麦、6V/6A、6VS/6AL、与6VS/6DL之间的双向电泳谱型极为相似,但与簇毛麦不同。在代换系、两个易位系和簇毛麦中检测到了特异蛋白组分16 KD/pI5.0,而在栽培小麦中未检测到该组分,这些结果表明16 KD/pI5.0蛋白可能定位于簇毛麦V染色体短臂上。
The total protein of leaves in cultivated wheat (AABBDD), chromosome substitution lines (6V / 6A), translocation lines (6VS / 6AL), (6VS / 6DL) the study. Over 350 protein components were detected in cultivated wheat, the substitution lines and two translocations, with molecular weights ranging from 10 to 110 KD and isoelectric points between 4.5 and 8.6. The cultivars of wheat, 6V / 6A, 6VS / 6AL, had a very similar two-dimensional electrophoretic pattern to 6VS / 6DL, but different from that of H. villosa. The specific protein component 16 KD / pI5.0 was detected in the lines of substitution, two translocation lines and cluster wool but not in cultivated wheat. These results indicate that the 16 KD / pI5.0 protein It may be located on the short arm of chromosome V in H. villosa.