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本研究用１２对引物组合对杂交水稻生产中广泛应用的优良杂交组合及其亲本（共１５个材料）进行了ＡＦＬＰ分析。通过优选，筛选出 ３对引物，即 Ｅ－ＡＣＴ／Ｍ·ＣＡＣ， Ｅ－ＡＡＣ／Ｍ－ＣＡＡ及 Ｅ－ＡＡＧ／Ｍ－ＣＡＡ，它们是本研究中用来区分所研究的 １５个材料所需的最少引物组合。然后根据所选用的１６个多态性ＤＮＡ条带的有无，把每个材料的ＤＮＡ指纹转换成由１和０组成的指纹数据。在此基础上，开发了一套应用软件。在实际应用中，将样品的扩增结果转换成指纹数据，由计算机辅助进行种子鉴定。
In this study, 12 pairs of primer combinations were used to analyze AFLP for the excellent hybrid combinations widely used in hybrid rice production and their parents (15 materials in total). Three pairs of primers, E-ACT / M-CAC, E-AAC / M-CAA and E-AAG / M-CAA, were selected by preference and were used in this study to distinguish the 15 materials studied Minimum primer combination. The DNA fingerprints of each material are then converted into fingerprint data consisting of 1 and 0 based on the presence or absence of the 16 polymorphic DNA bands selected. On this basis, developed a set of application software. In practical application, the result of sample amplification is converted into fingerprint data, and computer assisted seed identification.