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【目的】筛选适宜小麦幼胚培养的培养基和转基因受体材料的优良基因型,为小麦转化、遗传等提供依据。【方法】以7个春小麦品种(系)的幼胚为材料,取开花后12~16 d的幼胚进行培养,20 d后统计幼胚一步成苗数和愈伤组织绿苗分化数。【结果】小麦幼胚在C17、MS和YP三种培养基上都能诱导一次成苗,其中YP培养基一次成苗率最高(75%),其次是MS(33.13%),最低的是C17(23.34%),YP培养基一次成苗的效果明显优于其它两种培养基,不同基因型间的愈伤组织绿苗分化率差异明显。【结论】筛选出适宜小麦幼胚培养一次成苗的YP培养基和一次成苗和愈伤组织分化绿苗率都较高的材料新春9号,此材料可作为幼胚培养和转基因受体材料的优良基因型。
【Objective】 The objective of this study was to screen the excellent genotypes suitable for the culture of wheat immature embryos and the materials of transgenic recipient materials, and to provide the basis for wheat transformation and inheritance. 【Method】 The immature embryos of seven spring wheat cultivars (lines) were used as materials. Immature embryos 12 to 16 days after flowering were used for culture. After 20 days, the number of young embryos and the number of callus differentiation of green calluses were counted. 【Result】 Immature embryos of wheat were inoculated once on C17, MS and YP mediums. Among them, YP medium had the highest rate of primary seedling formation (75%), followed by MS (33.13%), and the lowest one was C17 (23.34%). The result of one seedling formation in YP medium was obviously better than the other two mediums. The differentiation rate of callus in different genotypes differed significantly. 【Conclusion】 The results showed that YP medium, which was suitable for primary immature embryo culture of wheat, and the material with the highest percentage of green shoots and the second callus, were screened for the immature embryo culture and transgenic receptor materials Good genotype.