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目的 萎缩性胃炎是癌前疾病之一 ,常为非萎缩性胃炎演变的后果。应用生物芯片技术观察萎缩性胃炎和非萎缩性胃炎胃黏膜组织基因表达谱的差异 ,探讨萎缩性胃炎发生的分子生物学机制。方法 连续登记在门诊接受胃镜检查的萎缩性胃炎和非萎缩性胃炎患者 2 2 7例 ,男 14 3例 ,女 84例 ,平均年龄 4 8.6岁 (16～ 72岁 )。胃镜检查时获取胃体和胃窦黏膜活检标本进行病理学检查 ,其余活检黏膜组织液氮冻存。根据病理学检查结果将冻存的组织标本分成萎缩性胃炎组和非萎缩性胃炎组 ,分别抽提两组组织的总RNA ,逆转录制备荧光cDNA探针 ,用含有 84 6 4条人类体细胞基因模板的表达谱cDNA芯片进行杂交实验。结果 与非萎缩性胃炎相比 ,萎缩性胃炎的活检胃黏膜组织中 16 5项基因的表达水平上调 2倍以上 ,4 6 0项的表达水平下调 5 0 %以上。表达下调者占 73.6 %。结论 基因表达谱生物芯片技术可以获得萎缩性胃炎胃黏膜组织基因在表达水平上改变情况的较全面的信息 ;萎缩性胃炎涉及多基因在表达水平上的改变。
Atrophic gastritis is one of the pre-cancerous diseases and is often the consequence of non-atrophic gastritis. Biochips technique was used to observe the difference of gene expression profile of gastric mucosa between atrophic gastritis and non-atrophic gastritis, and to explore the molecular biological mechanism of atrophic gastritis. Methods Two hundred and seventy-seven patients with atrophic gastritis and non-atrophic gastritis who were enrolled in the clinic underwent endoscopy were enrolled. There were 143 males and 84 females, with an average age of 4. 8.6 years (range, 16-72 years). Gastroscopy and gastric mucosal biopsy specimens obtained for pathological examination, the remaining biopsy mucosal cryopreservation liquid nitrogen. According to the results of pathological examination, the frozen tissue samples were divided into atrophic gastritis group and non-atrophic gastritis group, total RNA was extracted from the two groups respectively, and fluorescent cDNA probe was reverse transcribed. A total of 84 6 4 human somatic cells Gene template expression profile cDNA microarray hybridization experiments. Results Compared with non-atrophic gastritis, the expression of 16 5 genes in gastric mucosa of atrophic gastritis was up-regulated more than 2-fold and the expression level of 460 genes was reduced by more than 50%. Down-regulated expression accounted for 73.6%. Conclusion Gene expression microarray technology can obtain a more comprehensive information on the changes of gastric mucosal gene expression in atrophic gastritis. Atrophic gastritis involves in the expression of multiple genes.