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目的通过人群、家畜发热伴血小板减少综合征布尼亚病毒(severe fever with thrombocytopenia syndrome bunyavirus,SFTSV)及人粒细胞无形体病(human granulocytic anaplasmosis,HGA)感染状况调查,阐明庐江县是否存在SFTSV和HGA自然疫源地,为科学防治工作提供依据。方法收集分析2009-2013年SFTS和HGA病例流行病学资料。在全县选取1个有病例的镇,采用多阶段随机抽样方法 ,随机抽取病例附近2个村庄,采集自然人群血清100份,15只家畜血块和血清各1份。对血清标本采用双抗体夹心法检测SFTSV抗体和间接荧光法检测人粒细胞无形体病抗体免疫球蛋白G,对动物标本采用巢式聚合酶链反应(polymerase chain reaction,PCR)检测人粒细胞无形体16SrRNA基因和荧光定量反转录PCR检测SFTSV核酸。结果 2009-2013年,共报告9例SFTS和1例HGA,以中老年为主,女性多于男性,夏秋季为发病高峰。人HGA阳性率24%,SFTSV抗体阳性率0%。15份动物标本SFTSV抗体阳性6份,HGA 16SrRNA基因阳性9份,双阳性5份,SFTSV核酸和人粒细胞无形体抗体均阴性。结论该县农村地区存在SFTSV和HGA疫源地,人群和家畜中均有感染,家畜可能是该类疾病的宿主动物。需加强宣传、培训和快速诊断能力。
OBJECTIVE: To investigate the prevalence of SFTSV and human granulocytic anaplasmosis (HGA) in people with fever and thrombocytopenia syndrome (Bunyavirus) HGA natural foci for scientific prevention and control to provide the basis. Methods The epidemiological data of cases of SFTS and HGA from 2009 to 2013 were collected and analyzed. In the county selected a town with a case, the use of multi-stage random sampling method, randomly selected two villages near the case, collecting 100 copies of the serum of natural populations, 15 livestock blood clots and serum 1 copies. Serum samples were detected by double antibody sandwich ELISA SFTSV antibody and indirect fluorescent detection of human granulocyte Aphthous immunoglobulin G antibody, SFTSV nucleic acid was detected using the 16S rRNA gene and fluorescence quantitative reverse transcription PCR. Results A total of 9 cases of SFTS and 1 case of HGA were reported from 2009 to 2013, mainly in middle-aged and elderly people. There were more women than men and peak incidence in summer and autumn. Human HGA positive rate of 24%, SFTSV antibody positive rate of 0%. Six SFPSV antibody positive samples were obtained from 15 animal samples, 9 were positive for HGA 16S rRNA gene, 5 were double positive, SFTSV nucleic acid and non-human granulocyte antibody were all negative. Conclusion SFTSV and HGA foci are found in rural areas of the county. Both humans and livestock are infected, and livestock may be host animals for such diseases. Need to strengthen advocacy, training and rapid diagnosis.