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当免疫荧光技术已成为显示角膜中异常沉着的免疫球蛋白的良法时,为了测出角膜中 IgG 的正常沉着情况,必须选用适宜的组织制备法。以往用冰冻法保存角膜后再用低温控制器切片,也有用乙醇——醋酸或冻干后再用石蜡包埋切片。有人用直接染色法(一层),另有人用间接染色法(2层)。本文旨在研究:(1)比较同一眼球的各个部分用低温控制器和乙醇——醋酸固定的结果,(2)
When immunofluorescence has become a good method of displaying abnormally immunoglobulin in the cornea, appropriate tissue preparation must be used in order to determine the normal calm of IgG in the cornea. Cryosurgery used to save the cornea before cryogenic controller section, but also useful ethanol - acetic acid or freeze-dried and then paraffin embedded sections. Some people use direct staining (one), while others use indirect staining (two). The purpose of this paper is to study: (1) comparing the results of different parts of the same eye with a cryogenic controller and ethanol-acetic acid fixation, (2)