【摘 要】
：
This study examined the effects of ursolic acid (UA) on the proliferation and apoptosis of a human ovarian cancer cell line,CAOV3. The CAOV3 cells were cultured
【机 构】
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Department of Gynecology and Obstetrics,Xinhua Hospital of Hubei Province,Wuhan 430015,China“,”Depar
【出 处】
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华中科技大学学报（医学）（英德文版）
论文部分内容阅读
This study examined the effects of ursolic acid (UA) on the proliferation and apoptosis of a human ovarian cancer cell line,CAOV3. The CAOV3 cells were cultured in the RPMI 1640 media and treated with different concentrations of UA (0,10,20,40 μmol/L). The proliferation rate of the CAOV3 cells was determined by MTT assay. The apoptosis rate was measured by flow cytometry.ERK activity was detected by immunoprecipitation and the expressions of p-ERK1/2,MKP-1,Bax and Bcl-2 by West blotting. The results showed that the proliferation rate was significantly decreased in the cells treated with UA as compared with that in the non-treated cells (P＜0.05). The intracellular ERK activity and p-ERK1/2 expression were also reduced in the UA-treated cells,while the MKP-1 expression was elevated. Moreover,the apoptosis was found in the CAOV3 cells exposed to UA;the Bax expression was increased and the Bcl-2 expression decreased. The apoptosis rate in the UA-treated ceils was much higher than that in the non-treated cells (P＜0.05). It is concluded that UA can inhibit the proliferation of CAOV3 cells by suppressing the ERK activity and the expression of p-ERK1/2. And it can also induce the apoptosis of the CAOV3 cells by up-regulating the Bax expression and down-regulating the Bcl-2 expression.
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