目的探讨癌基因Ｂｃｌ２，Ｃｍｙｃ的表达变化与胃癌生物学行为和细胞凋亡的关系．方法采用免疫组化ＬＳＡＢ法，检测６０例原发性胃癌组织（男３８例，女２２例，年龄３７岁～７５岁）癌基因Ｂｃｌ２，Ｃｍｙｃ蛋白的表达变化；在普通光学显微镜下对受检组织的ＨＥ片进行形态学测量和凋亡细胞计数．结果受检组织６０例中，Ｃｍｙｃ阳性表达３７例（６２％），其表达与分化程度和临床分期呈显著性相关，且Ｃｍｙｃ阳性组织细胞凋亡指数（０７±０３）明显高于阴性组织（０３±０２）；所检标本中，Ｂｃｌ２阳性表达率为６８％（４１／６０），其中高分化胃癌的阳性表达率明显高于低分化胃癌（３２％ｖｓ９％）（Ｐ＜００５），阳性Ｂｃｌ２组织与阴性Ｂｃｌ２组织比较，前者凋亡指数明显低于后者（０４±０３ｖｓ０９±０５）（Ｐ＜００５）．结论Ｃｍｙｃ与Ｂｃｌ２基因的异常表达是胃癌生物学行为的重要影响因素，二者在胃癌形成过程中均起着一定的作用，并对细胞增生与凋亡有重要调节作用． Objective To investigate the relationship between the expression of oncogenes Bcl  2 and Cmyc and the biological behavior and apoptosis of gastric cancer. Methods Immunohistochemical LSAB method was used to detect the expression of Bcl2 and Cmyc protein in 60 cases of primary gastric cancer (38 males and 22 females, aged 37 to 75 years); The morphological measurements and apoptotic cell counts were performed on the HE slices of the examined tissues. RESULTS: Of the 60 cases examined, 37 cases (62%) were positive for C-myc. The expression of C-myc was significantly correlated with the degree of differentiation and clinical stage, and the apoptosis index of C-myc-positive tissue (07±0). 3) significantly higher than the negative tissue (0.3 ± 0  2); Bcl  2 positive expression rate in the specimens was 68% (41/60), of which the positive expression rate of well-differentiated gastric cancer was significantly higher than that of poorly differentiated Gastric cancer (32% vs 9%) (P<005), positive Bcl2 tissue and negative Bcl2 tissue, the former apoptotic index was significantly lower than the latter (04±03 vs 09±0) 5) (P<005). Conclusion The abnormal expression of Cmyc and Bcl2 genes is an important influencing factor in the biological behavior of gastric cancer. Both of them play a certain role in the formation of gastric cancer and play an important role in regulating cell proliferation and apoptosis.