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目的探讨血液病患者血液中肺炎支原体和发酵支原体的分离检出。方法分别对101例确诊为血液病患者和65名非血液病对照者的血液标本进行肺炎和发酵支原体分离培养和聚合酶链反应鉴定,阳性标本进一步经电镜确认。结果101例血液病患者血液标本中共计检出肺炎和发酵支原体17例(16.8%),其中肺炎支原体9例(8.9%),发酵支原体8例(7.9%),体检对照组血液中未检出肺炎和发酵支原体(0.0%),组间差异有统计学意义(P<0.01)。结论血液病患者血液中肺炎支原体和发酵支原体的检出显著高于非血液病对照组,确切机制有待进一步探讨。
Objective To investigate the separation and detection of Mycoplasma pneumoniae and Mycoplasma fermentum in the blood of patients with hematological diseases. Methods The blood samples of 101 patients diagnosed as hematological diseases and 65 non-hematological controls were respectively isolated from pneumonia and Mycoplasma fermentatively and identified by polymerase chain reaction. The positive specimens were confirmed by electron microscopy. Results A total of 17 cases (16.8%) of pneumonia and mycoplasma were detected in the blood samples of 101 cases of hematological diseases, including 9 cases of Mycoplasma pneumoniae (8.9%) and 8 cases of Mycoplasma fermentum (7.9%). No blood was detected in the control group Pneumonia and Mycoplasma fermentum (0.0%), the difference was statistically significant (P <0.01). Conclusion The detection of Mycoplasma pneumoniae and Mycoplasma mycoplasma in the blood of patients with hematological diseases was significantly higher than that of non-hematological controls. The exact mechanism remains to be further explored.