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目的:探讨2009年新型甲型H1N1流感病毒的非结构蛋白(NS)基因进化规律。方法:从NCBI下载2009年新型甲型H1N1流感病毒以及北美、欧洲、亚洲地区以往流行的甲型H1N1流感病毒NS基因序列,利用Molecular Evolutionary Genetics Analysis version 4.0(MEGA4.0)软件对所选序列进行基因进化分析,并用NJ法构建进化树;对2009年新型甲型H1N1流感病毒NS基因核苷酸序列同源性及编码蛋白氨基酸序列进行分析。结果:2009年新型甲型H1N1流感病毒NS基因来源于猪A/H1N1流感病毒,与2005～2007年猪A/H1N1流感病毒具有较高的同源性(97.5%～97.6%),与1930～2007年猪A/H1N1流感病毒具有明显的时间进化关系;其重要抗原及拮抗宿主抗病毒能力的氨基酸位点基本没有变异。结论:2009年新型甲型H1N1流感病毒NS基因来源于猪A/H1N1流感病毒,NS基因编码蛋白拮抗宿主抗病毒能力并没有改变。
Objective: To investigate the evolution of the non-structural protein (NS) gene of new type A H1N1 influenza virus in 2009. Methods: The NS (H1N1) virus sequence of 2009 new type A (H1N1) virus and the influenza A (H1N1) virus that was prevalent in North America, Europe and Asia were downloaded from NCBI. The selected sequences were analyzed by Molecular Evolutionary Genetics Analysis version 4.0 (MEGA 4.0) The phylogenetic tree was constructed and the phylogenetic tree was constructed by NJ method. The homology of the NS gene of the new type A H1N1 influenza virus in 2009 and the amino acid sequence of the encoded protein were analyzed. Results: In 2009, the NS gene of the new type A H1N1 influenza virus was derived from the swine A / H1N1 influenza virus and had high homology (97.5% -97.6%) with the swine A / H1N1 influenza virus from 2005 to 2007, In 2007, the swine A / H1N1 influenza virus had a significant evolutionary relationship in time. There was almost no variation in the major antigens and the amino acid sites that antagonized the antiviral ability of the host. Conclusion: In 2009, the NS gene of the new type A H1N1 influenza virus was derived from the swine A / H1N1 influenza virus, and the NS gene-encoded protein did not change the antiviral ability of the host.