银杏内酯B对小鼠胚胎着床过程中滋养层和蜕膜组织超微结构的影响

来源 :生殖与避孕 | 被引量 : 0次 | 上传用户:tutu321
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:观察银杏内酯B(GB)抗小鼠胚胎着床的组织形态学与超微结构变化特征。方法:将25只未经产成年雌性昆明小鼠随机分成5组,每组5只,于妊娠第2日右侧子宫角注射不同剂量的GB(2 mg/kg、4 mg/kg、6 mg/kg、8 mg/kg、10 mg/kg)作为实验侧,左侧子宫角注射等量的生理盐水作为阴性对照侧。另取3只孕鼠不作任何处理,作为空白对照组。于妊娠第8日处死小鼠,取子宫组织,观察GB对滋养层细胞和子宫蜕膜细胞组织形态学和超微结构的影响。结果:妊娠第8日,实验侧小鼠胚胎发育不良,子宫壁充血,胚胎总数显著少于阴性对照侧。光镜下观察到实验侧蜕膜相对较薄、绒毛间隙有白细胞浸润、巨细胞结构松散,并有部分蜕膜细胞发生变性和坏死;电镜下观察到实验侧滋养层细胞胞质电子密度减低、出现小空泡、核形态发生改变、蜕膜细胞胞质内溶酶体增多、线粒体发生肿胀,而且随着GB剂量增大(≥8 mg/kg),滋养细胞胞质出现大量溶酶体、核固缩、碎裂,蜕膜细胞胞质亦出现大量溶酶体、核固缩、碎裂,并出现核膜溶解。结论:GB可造成绒毛滋养细胞及蜕膜细胞超微结构的改变,破坏胚胎赖以生存与发育的内环境,从而干扰胚胎着床。 Objective: To observe the histological and ultrastructural changes of ginkgolide B (GB) anti-mouse embryo implantation. Methods: Twenty-five female Kunming mice were randomly divided into 5 groups with 5 mice in each group. Different doses of GB (2 mg / kg, 4 mg / kg, 6 mg) / kg, 8 mg / kg, 10 mg / kg) as the experimental side, the left uterine horn injection of saline as a negative control side. Another three pregnant rats without any treatment, as a blank control group. Mice were sacrificed on the 8th day of gestation and the uterus was taken for observation of the effect of GB on the histomorphology and ultrastructure of trophoblast cells and uterine decidual cells. Results: On the 8th day of gestation, the experimental mice developed hypoplastic embryos, uterine wall hyperemia, and the total number of embryos was significantly less than that of the negative control. Under the light microscope, the experimental decidua was relatively thin, leukocyte infiltration was found in the villus space, the giant cell structure was loose, and some decidual cells were degenerated and necrotic. The cytoplasmic electron density was reduced under the electron microscope, Small vacuoles appeared, nuclear morphology changed, lysosomes in the cytoplasm of decidual cells increased and mitochondria swollen. With the increase of GB dose (≥8 mg / kg), a large number of lysosomes appeared in the cytoplasm of trophoblast cells, Nuclear condensation, fragmentation, decidual cell cytoplasm also appeared a large number of lysosomes, nuclear pyknosis, fragmentation, and nuclear membrane dissolution. Conclusion: GB can cause changes in the ultrastructure of trophoblasts and decidual cells, disrupt the inner environment of the embryo that depends on its survival and development, and interfere with embryo implantation.
其他文献
低密度脂蛋白(LDL)氧化作用在动脉硬化的发生中起着重要作用。但这种作用可被药物和营养素有效地抑制。业已观察到普罗布考(probucol)具有降低高密度脂蛋白(HDL)的效用,并提出了长期大剂量
吲达帕胺治疗高血压对左室重量的影响(附42例报告)湖南省怀化地区第一人民医院(418000)梁北南谭军邓兰芳高血压伴左室肥厚者发生充血性心衰和猝死的危险性显著增高,且是冠心病的易患因素之
“入局斗牌,必先炼品,品宜镇静,不宜躁率,得牌勿骄,失牌勿吝,顺时勿喜,逆时勿愁,不形于色,不动乎声,浑涵宽大,品格为贵,尔雅温文,斯为上乘。”这段麻将的旨意与精神,有说是源于三宝太监郑和,不过至今尚未考证确实,能肯定的是——这段话在数百年前就已经流传了。  古代中国,麻将似乎是很上台面的斯文运动,可实际上,长期以来,麻将被国人视为赌博工具,不屑者对之嗤之以鼻,迷恋者称之小赌怡情,当然也有打麻将输
论文自我剽窃亦称变相重复发表,多年来屡见不鲜。为了帮助作者特别是年轻作者认识论文自我剽窃行为及其危害性,维护学术的尊严和诚信,现将《编辑学报》发表的“科技论文自我
本文以字体杂糅现象的成因为视角,将字体杂糅分为『技法不纯』、『有意为之』和既『技法不纯』又『有意为之』三种情况,并通过列举篆隶、篆楷、楷隶、行楷杂糅的典型作品来具
The plasma-surface interaction is an important task in the nuclear fusion research. The interaction of plasma on graphite and the coated graphite has been stud
本文侧重从利益均衡的角度,提出了在当前数字权益管理(DRM)系统中存在的利益不均衡问题。在现有技术基础上提出基于第三方监管、支持数字许可证二次转发的一种新的数字版权管
目的了解南阳市MSM人群AIDS流行趋势及相关行为学信息,为制定艾滋病防治措施提供科学依据。方法通过网络QQ群、打电话、发短信、同伴教育等形式招募调查对象,对调查对象开展
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
细菌内毒素国家标准品是作为基准物质来标定鲨试剂灵敏度的。它是细菌内毒素检查法标准化和规范化的关键,关系到整个实验结果的准确性和可靠性,因此它的作用与地位是可想而知的