为了使单纯疱疹病毒1型(HSV-1)的BamHI C片段发生突变,利用DNA重组技术将胸腺嘧啶激酶基因-小Mu噬菌体DNA系统(TK—mM)随机插入到质粒pRB 132中的HSV-1 BamHI C片段内,用DNA斑点杂交法得到6个在BamHI C片段上有TK-mM插入的重组质粒。限制性核酸内切酶的分析结果表明,由于TK—mM的插入,BamHI C片段发生突变,而TK—mM在6个重组质粒中的插入位点也是各不相同的。 To mutate the BamHI C fragment of herpes simplex virus type 1 (HSV-1), a thymidine kinase gene-small Mu phage DNA system (TK-mM) was randomly inserted into HSV-1 in plasmid pRB 132 Within the BamHI C fragment, six recombinant plasmids containing the TK-mM insert on the BamHI C fragment were obtained by DNA dot blot hybridization. Analysis of the restriction endonuclease showed that the BamHI C fragment was mutated due to the insertion of TK-mM, whereas the insertion sites of TK-mM in the six recombinant plasmids were also different.