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We studied the effect of agar concentration inmedia on callus induction rate and green plant-let regeneration frequency in rice.Materialswere F_3 generation of indica/indica or indica/japonica,which were 96E76(Hei’e/Zhaiye- qing 8),96E80[(IR 24/Guanglu＇ai 4//Zhongnan’ai)/Yifengzao],96E86(Zhong- munong 9/Zhaiyeqing 8).The induction mediaused was M8+2mg/L 2,4-D,and agar con-centrations were 0.6％,0.8％,and 1.0％,respectively.Regeneration media was MS+2mg/L KT+0.5mg/L IAA+0.5mg/LNAA,and agar concentrations were 0.6％ and1.0％.Results indicated that the calli induc-
We studied the effect of agar concentration in media on callus induction rate and green plant-let regeneration frequency in rice. Materialswere F_3 generation of indica / indica or indica / japonica, which were 96E76 (Hei’e / Zhaiye-qing 8), 96E80 [ (IR 24 / Guanglu’ai 4 // Zhongnan’ai) / Yifengzao], 96E86 (Zhong-munong 9 / Zhaiyeqing 8). The induction media used was M8 + 2 mg / L 2,4-D, and agar con-centrations were 0.6%, 0.8%, and 1.0%, respectively.Regeneration media was MS + 2 mg / L KT + 0.5 mg / L IAA + 0.5 mg / L NAA, and agar concentrations were 0.6% and 1.0% .Results indicated that the calli induc -