论文部分内容阅读
目的 :研究雄激素受体 (AR)、雌激素受体 (ER)及孕激素受体 (PR)在前列腺增生症(BPH)患者前列腺组织中的分布及 AR的含量 ,探讨性激素受体在 BPH发病中的作用。方法 :应用免疫组织化学 ABC染色法结合图像分析技术检测 36例 BPH组织中的 AR、ER、PR。结果 :AR、ER的阳性率及染色强度在基质显著高于腺上皮 ,腺上皮 AR与 ER染色强度呈正相关 ;基质中 AR染色强度的增加主要表现为 AR阳性细胞数量的增加 ;年龄愈大 ,尿路梗阻愈轻 ,其腺上皮 AR染色强度愈高 ;基质中 ,纤维肌型增生者 AR染色强度高于纤维肌腺瘤样型增生者 ;AR阳性细胞的吸光度值与染色强度呈正相关 ;基质和腺上皮中 AR含量无显著性差异 (P >0 .0 5)。结论 :BPH前列腺基质和腺上皮中 AR与 ER的分布不等 ,其阳性细胞含量与患者年龄、尿路梗阻程度及病理类型无相关性。图像分析技术克服了半定量法的主观误差 ,结果精确可靠 ,它为 BPH生物学特性的研究提供了一种新型的定量指标。
Objective: To study the distribution and AR content of androgen receptor (AR), estrogen receptor (ER) and progesterone receptor (PR) in prostatic tissue of patients with benign prostatic hyperplasia (BPH) The role of the disease. Methods: Immunohistochemical ABC staining combined with image analysis was used to detect AR, ER and PR in 36 cases of BPH. Results: The positive rate and staining intensity of AR and ER were significantly higher in the stroma than in the glandular epithelium. There was a positive correlation between AR and ER staining intensity. The AR staining intensity in the stroma mainly showed an increase in the number of AR positive cells. The lighter the urinary tract obstruction, the higher the intensity of AR staining in the glandular epithelium. In the stroma, AR staining intensity of fibromuscular hyperplasia was higher than that of fibroadenoma-like hyperplasia. The absorbance value of AR positive cells was positively correlated with the staining intensity. And glandular epithelial AR content was no significant difference (P> 0.05). CONCLUSION: The distribution of AR and ER in prostate stroma and glandular epithelium of BPH is not equal. The positive cell content has no correlation with patient’s age, urinary tract obstruction and pathological type. Image analysis overcomes the subjective error of semi-quantitative method, and the result is accurate and reliable. It provides a new quantitative index for the study of biological characteristics of BPH.