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用水稻抗白叶枯病基因近等基因系材料和转基因系材料,研究了水稻—白叶枯病菌互作中,水稻防卫基因pal与chs的转录特征。Northern检测结果显示,在与白叶枯病菌非亲和性互作中,水稻pal基因和chs基因的转录均受到白叶枯病菌的诱导;而亲和性互作中,水稻中这两种基因的转录均不受到诱导或只是受到很微弱的诱导。表明由pal和CHS调控的苯丙烷核心反应的启动,产物的合成,有助于增强水稻对白叶枯病菌的抗性。实验结果显示:互作中水稻PAL及CHS编码基因是否受到白叶枯病菌的诱导,不仅与水稻中抗病基因有关,还与抗病基因的种类有关。表明水稻PAL及CHS编码基因在互作中是否受到诱导转录,取决于其水稻功能性抗病基因产物对白叶枯病菌的专化性识别。
The rice bacterial leaf blight resistance gene near-isogenic lines and transgenic lines were used to study the transcriptional characteristics of rice defensive genes pal and chs during the interaction between rice bacterial blight and bacterial blight. Northern analysis showed that the transcription of pal genes and chs genes was induced by Xanthomonas oryzae in the non-affinity interaction with B.cerevisiae, while in affinity interactions, the two genes in rice Of the transcripts were not induced or only very weakly induced. This indicates that the initiation of palmitate and CHS-regulated core reactions of phenylpropanoids and the synthesis of the product are helpful to enhance the resistance of rice to Xanthomonas oryzae. The results showed that whether PAL and CHS-encoding genes in rice were induced by Xanthomonas oryzae were not only related to disease-resistance genes in rice, but also to the types of disease-resistance genes. The results indicated that whether rice PAL and CHS coding genes were induced to be transcribed in the interaction depends on the specific recognition of the bacterial resistance genes of rice to the bacterial leaf blight.