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目的比较飞行质谱法、肌钙蛋白Ⅰ(TnI)快速检测法在早期急性心肌梗死(AMI)诊断中的作用。方法选择胸痛发作2h之内的早期AMI(A1组)患者45例,正常对照组45例。利用飞行质谱法测定所有血清,利用TnI快速检测法测定AMI患者的血清。再将病历齐全的同时用飞行质谱法和TnI快速检测法测定的患者进行比较。结果①A1组患者与正常人之间质荷比为8149.84和5910.00。这两种蛋白质的差异呈显著性。A1组患者在质荷比8149.84的蛋白质呈低表达;而在质荷比5910.00的蛋白质呈明显高表达,可作为诊断早期AMI的血清标志物。②飞行质谱法的敏感性是000000E-M8,特异性是0.008,飞行质谱法对早期AMI的诊断的敏感性和特异性都明显优于TnI快速检测法。结论飞行质谱方法灵敏度和特异性高,可快速、准确诊断早期AMI,优于TnI快速检测法。
Objective To compare the diagnostic value of rapid mass spectrometry and TnI in the diagnosis of early acute myocardial infarction (AMI). Methods 45 cases of early AMI (A1 group) and 45 cases of normal control group were selected within 2 hours after onset of chest pain. All sera were determined by flight mass spectrometry and sera were measured by rapid TnI assay in patients with AMI. And then the medical records at the same time by flight mass spectrometry and TnI rapid detection of patients were compared. Results ① The mass-to-charge ratio of patients in A1 group and normal people was 8149.84 and 5910.00. The difference between the two proteins was significant. Patients in A1 group showed a low expression of the protein with a mass-to-charge ratio of 8149.84, while a protein with a high mass-to-mass ratio of 5910.00 was highly expressed, which could be used as a serum marker in the diagnosis of early AMI. ② The sensitivity of flight mass spectrometry was 000000E-M8 and the specificity was 0.008. The sensitivity and specificity of flight mass spectrometry in the diagnosis of early AMI were significantly better than that of TnI. Conclusion The method of flight mass spectrometry has high sensitivity and specificity, which can quickly and accurately diagnose early AMI and is superior to TnI rapid detection.