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建立了一种新的测定Fenton反应所产生的羟自由基的方法 ,苯基荧光酮 (Phenylfluorone ,简称本芴酮 )与Fenton试剂作用后 ,使其荧光大大降低 ,其最大激发波长和发射波长分别为 50 0nm和 42 0nm ,基于苯基荧光酮在反应前后的荧光变化 ,即可间接地测定羟自由基的产生量。同时 ,通过清除率实验反证了该方法的可靠性 ,利用顺磁共振法测定苯基荧光酮加入前后的波谱变化来进一步证实该方法的正确性。化学测定过程均在国产仪器下完成 ,具有较高的灵敏度。可推广为一种作为寻找羟自由基清除剂的方法 ,也可作为医学上部分药品性能检验的一种方法
A new method for the determination of hydroxyl radical generated by Fenton reaction was established. After phenylfluorone (PGF) reacted with Fenton reagent, its fluorescence decreased greatly. The maximum excitation wavelength and emission wavelength of Phenylfluorone 50 0nm and 42 0nm, based on the fluorescence of phenylfluorone before and after the change in the fluorescence, you can indirectly determine the amount of hydroxyl radicals. At the same time, the reliability of the method was verified by the clearance rate experiment, and the correctness of the method was further confirmed by the paramagnetic resonance spectroscopy before and after the addition of phenylfluorone. Chemical determination process are done under domestic instruments, with high sensitivity. Can be promoted as a method of looking for hydroxyl radical scavengers, but also as a method of medical performance testing of some drugs