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为了了解咖啡因对胚胎及新生时期男性生殖系统的影响，采用低（０３ｍｍｏｌ／Ｌ）、中（０６ｍｍｏｌ／Ｌ）、高（１２ｍｍｏｌ／Ｌ）浓度咖啡因体外培养ＳＤ孕１８ｄ胎鼠、０ｄ及４ｄ乳鼠睾丸组织块，用ＨＥ染色及放射自显影方法检测咖啡因对睾丸内生殖细胞数量及其摄取３Ｈ－ＴｄＲ的影响。结果：１８ｄ胎鼠睾丸培养组织内生殖细胞数量及３Ｈ－ＴｄＲ的摄取受咖啡因影响较少；中等浓度的咖啡因在培养３周后，生殖细胞的数量、３Ｈ－ＴｄＲ的摄取才降低；而高浓度的咖啡因在培养２周后，检测指标已有下降，３周更明显。生殖细胞数量的减少往往伴随３Ｈ－ＴｄＲ摄取的降低，由此推测高浓度咖啡因长时间培养后使生殖细胞数量减少可能与抑制生殖细胞摄取３Ｈ－ＴｄＲ，从而干扰ＤＮＡ的合成有关。
In order to understand the effect of caffeine on the reproductive system of embryos and newborn men, SD pregnant female rats were cultured with low (0.3 mmol / L), (0.6 mmol / L) and high (1.2 mmol / L) Fetal rat, 0d and 4d neonatal testes, HE staining and autoradiography were used to determine the effect of caffeine on the number of testicular germ cells and their uptake of 3H-TdR. Results: The quantity of germ cells and the uptake of 3H-TdR in testis tissue of 18-day embryos were less affected by caffeine. The number of germ cells and the 3H-TdR uptake were lower after moderate concentration of caffeine was cultured for 3 weeks High concentrations of caffeine after 2 weeks of culture, test indicators have decreased, 3 weeks more obvious. The decrease of germ cell number is often accompanied by the decrease of 3H-TdR uptake. Therefore, it is speculated that the decrease of germ cell number after prolonged culture with high concentration of caffeine may be related to inhibition of uptake of 3H-TdR by germ cells and thus interference with DNA synthesis.