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目的观察糖尿病肾病大鼠血糖、尿蛋白的变化以及消渴颗粒剂对其肾小球形态的影响。方法采用 3/ 4肾切除 ,腹腔一次性注射链脲佐菌素 (streptozotocin ,STZ)复制大鼠糖尿病肾病模型。将动物分为模型组、消渴颗粒剂治疗组 (中药治疗组 )、阳性药对照组和假手术组。每组大鼠于注射STZ后 1、2、3、4、5周尾尖取血测定血糖和 2 4h尿蛋白。各组大鼠于注射STZ后 2、6周分别杀检 ,进行肾脏形态学观察。结果注射STZ后 6周 ,模型组大鼠有不同程度的肾小球硬化 ,中药治疗组上述病变明显轻于模型组。注射STZ后 1、2、3、4、5周模型组血糖明显高于假手术组 (P <0 0 5 ) ,中药治疗组和阳性药对照组与模型组同期比较血糖明显下降。 2 4h尿蛋白模型组大鼠于造模后第 1、2、3、4、5周明显高于假手术组 ;中药治疗组在 1、2、3周明显低于模型组。模型组血糖和 2 4h尿蛋白呈正相关。结论采用此方法成功的复制了大鼠糖尿病肾病模型 ,糖尿病肾病大鼠血糖增高可能引起蛋白尿 ,消渴颗粒剂治疗糖尿病肾病的途径之一是降低大鼠血糖 ,减轻蛋白尿 ,从而改善肾小球病变。
Objective To observe the changes of blood glucose and urinary protein in rats with diabetic nephropathy and the effect of Xiaoke granule on glomerular morphology. METHODS: Rats with diabetic nephropathy were treated with 3/4 nephrectomy and intraperitoneal injection of streptozotocin (STZ). Animals were divided into model group, Xiaoke granule treatment group (Chinese medicine treatment group), positive drug control group and sham operation group. Blood glucose and 24 hours of urinary protein were measured at 1, 2, 3, 4 and 5 weeks after the injection of STZ in each group of rats. Rats in each group were killed 2 or 6 weeks after STZ injection for renal morphology observation. RESULTS: Six weeks after the injection of STZ, rats in the model group had different degrees of glomerulosclerosis. The lesions in the Chinese medicine treatment group were significantly lighter than those in the model group. After 1,2,3,4,5 weeks after injection of STZ, the blood glucose level in the model group was significantly higher than that in the sham group (P < 0.05). The blood glucose levels of the Chinese medicine treatment group and the positive drug control group were significantly lower than those of the model group. The rats in the 24 h urine protein model group were significantly higher than those in the sham operation group at 1, 2, 3, 4 and 5 weeks after model establishment. The Chinese medicine treatment group was significantly lower than the model group at 1, 2 and 3 weeks. There was a positive correlation between blood glucose and 24 h urinary protein in the model group. Conclusions This method successfully replicates diabetic nephropathy model in rats. Diabetic nephropathy rats may cause hyperglycemia proteinuria, Xiaoke granules treatment of diabetic nephropathy is one way to reduce blood glucose in rats, reduce proteinuria, thereby improving renal small Ball lesions.