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本研究以海陆渐渗系13-1与陆地棉辽棉12构建的F2群体为材料,利用4 500对中具有多态性的84对SSR引物进行群体间分析。研究结果显示:在p<0.05显著水平上,共有19个分子标记表现偏分离,占总标记数的25.3%,其中有8个标记偏向父本辽棉12,占偏分离标记总数的42.1%;10个标记偏向母本13-1,占偏分离标记总数的52.63%;1个标记偏向杂合体,占偏分离标记总数的5.27%。这些标记在图谱上有两种分布形式,分别为成簇分布和孤立分布。在7条不同的染色体上均发现偏分离标记,其中在1染色体上发现1个热点区域。本研究分析了产生偏分离的原因,并讨论偏分离标记对QTL定位的影响。
In this study, F2 populations of Haolunshan 13-1 and Gossypium hirsutum 12 were used as materials, and 84 SSR primers with 4 500 pairs of polymorphism were used for population analysis. The results showed that at the significant level of p <0.05, a total of 19 molecular markers were segregated, accounting for 25.3% of the total number of markers, of which 8 markers were biased towards male parents 12, accounting for 42.1% of the total segregation markers. Ten markers were biased towards female parent 13-1, accounting for 52.63% of the total number of segregation markers. One marker was biased toward hybrids, accounting for 5.27% of the total number of segregation markers. There are two kinds of distributions of these markers on the map, which are clustered distribution and isolated distribution. Partial segregation markers were found on seven different chromosomes, of which one hot spot was found on chromosome 1. This study analyzed the causes of partial segregation and discussed the effect of partial segregation markers on QTL mapping.