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目的:探讨应用四环素调控长链非编码RNA TUC338在膀胱癌细胞内的表达及抗肿瘤作用。方法:将针对TUC338的sh RNA序列插入到四环素诱导系统的下端,构建四环素诱导的TUC338 sh RNA并转染细胞。应用实时荧光定量PCR检测细胞内TUC338的表达水平,采用CCK8和半胱天冬酶3酶联免疫吸附测定法的方法分别检测细胞的增殖和凋亡。结果:四环素诱导的TUC338 sh RNA可以降低TUC338的表达,抑制膀胱癌细胞增殖和促进膀胱癌细胞的凋亡。结论:通过构建四环素基因表达调控系统,可精确控制TUC338在膀胱癌细胞内表达,并发挥杀伤肿瘤细胞功能,为进一步构建特异、高效的膀胱癌合成生物治疗器件提供理论基础。
Objective: To investigate the expression and antitumor effect of tetracycline-regulated long-term non-coding RNA TUC338 in bladder cancer cells. Methods: The sh RNA sequence targeting TUC338 was inserted into the lower end of tetracycline-induced system to construct tetracycline-induced TUC338 sh RNA and transfected into cells. The expression of TUC338 in cells was detected by real-time fluorescence quantitative PCR. The proliferation and apoptosis of cells were detected by CCK8 and caspase 3 enzyme-linked immunosorbent assay (ELISA). Results: Tetracycline-induced TUC338 shRNA could reduce the expression of TUC338, inhibit the proliferation of bladder cancer cells and promote the apoptosis of bladder cancer cells. CONCLUSION: The tetracycline gene expression regulation system can control the expression of TUC338 in bladder cancer cells accurately and exert the function of killing tumor cells, providing a theoretical basis for further constructing a specific and efficient synthetic biological therapy device for bladder cancer.