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目的检测血浆presepsin在新生儿脓毒症患者外周血中的表达水平并探讨其意义。方法选取2014年1月-2015年10月本院NICU收治的49例脓毒症新生儿为病例组,选择同期产科出生的102例健康新生儿为对照组。病例组采用新生儿危重病例评分(NCIS)分级为极危重组、危重组和非危重组。收集所有对象空腹血,分别采用化学发光酶免疫法和酶联免疫荧光法检测血浆presepsin和降钙素原(PCT)水平,并进行比较分析。结果脓毒症组血浆presepsin和PCT水平均显著高于对照组,差异有统计学意义(P<0.01);其中脓毒症组中极危重组、危重组和非危重组血浆presepsin水平依次下降,而PCT在3组两两比较中除了非危重组与极危重组比较差异有统计学意义外(P<0.01),其余差异均无统计学意义(P>0.05)。结论新生儿脓毒症患者血浆presepsin水平显著增高,且与NCIS分级相关,是一种较好的预测脓毒症的生物标志物,有助于新生儿脓毒症的诊断。
Objective To detect the expression of plasma presepsin in peripheral blood of neonatal sepsis patients and to explore its significance. Methods From January 2014 to October 2015, 49 neonates with sepsis admitted to NICU in our hospital were selected as the case group. 102 healthy newborn infants were selected as the control group. The case group was graded as critically ill, critically ill and non-critically ill using the Neonatal Critical Illness Scale (NCIS). Fasting blood samples were collected from all subjects. Plasma presepsin and procalcitonin (PCT) levels were measured by chemiluminescence enzyme-linked immunosorbent assay and enzyme-linked immunosorbent assay, respectively. Results The levels of plasma presepsin and PCT in sepsis group were significantly higher than those in control group (P <0.01), and the levels of presepsin in critically ill, critically ill and non-critically ill groups were decreased in sepsis group, However, there was no significant difference in PCT between the two groups (P <0.01) except non-critically ill group and critically ill patients (P> 0.05). Conclusion The plasma presepsin level is significantly higher in neonates with sepsis and is correlated with NCIS classification. It is a good biomarker for predicting sepsis and is helpful for the diagnosis of neonatal sepsis.