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研究显示,山莨菪碱预处理不改变高钾引起的兔主动脉环收缩,但可明显减弱去甲肾上腺素(noradrenaline,NA)、组织胺或5-羟色胺引起的收缩,且其减弱作用不受去除血管内皮影响。本实验观察了几种钾通道阻断剂对山莨菪碱松弛NA预收缩的兔主动脉环的影响。结果表明,1、3、10μmol/L山莨菪碱作用8min,可使0.01μmol/LNA预收缩的兔主动脉环松弛(P<0.01)。10mmol/LCsCl、1mmol/L4-氨基吡啶、10μmol/LBaCl2、10μmol/L格列本脲、3μmol/Lcharybdotoxin和3μmol/L蜂毒明肽分别与O.01μmol/LNA同时加入,可增强后者收缩兔主动脉环的作用(P<0.01)。10、30mmol/LCsCl或10、30mmol/L4-氨基吡啶存在时,10μmol/L山莨菪碱对NA预收缩的兔主动脉环的松弛作用减弱,松弛率与对照组比较分别有极显著差异(P<0.01);10、30μmol/LBaCl2,10、30μmol/L格列本脲,3μmol/Lcharybdotoxin或3μmol/L蜂毒明肽存在时,山莨菪碱对NA预收缩的兔主动脉环的松弛作用不受影响(P>0.05)。本研究表明,电压激活的钾通道阻断剂抑制山莨菪碱松弛NA预收缩的兔主动脉平滑肌,初步提示血管平滑肌细胞膜上电压激活的钾通道参与山莨菪碱扩血管作用。
Studies have shown that anisodamine pretreatment does not change the contraction of rabbit aortic rings caused by high potassium, but can significantly reduce noradrenaline (NA), histamine or serotonin-induced contraction, and its attenuation is not affected Remove vascular endothelial effects. This experiment observed several potassium channel blockers of anisodamine relaxation of NA precontracted rabbit aortic rings. The results showed that 1, 3, 10μmol / L anisodamine could relax the aortic rings precontracted by 0.01μmol / LNA for 8min (P <0.01). 10mmol / L HCl, 1mmol / L 4-aminopyridine, 10μmol / LBaCl2, 10μmol / L glyburide, 3μmol / Lcharybdotoxin and 3μmol / L bevacizumab were added with 0.01μmol / Aortic rings (P <0.01). In the presence of 10, 30mmol / L HCl or 10mmol / L 4-aminopyridine, the relaxation of 10μmol / L anisodamine attenuated the precontracted rabbit aortic rings and the relaxation rate was significantly different from that of the control group <0.01). In the presence of 10, 30μmol / LBaCl 2, 10, 30μmol / L glyburide, 3μmol / Lcharybdotoxin or 3μmol / L bepindomin, the relaxation effect of anisodamine on the precontracted rabbit aortic rings was not Affected (P> 0.05). This study shows that voltage-activated potassium channel blockers inhibit anisodamine relaxation NA precontracted rabbit aortic smooth muscle, initially suggesting that the voltage-activated potassium channel in the vascular smooth muscle cell membrane involved in anisodamine vasodilation.