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目的 建立结肠癌肝转移的动物模型 ,用于肿瘤转移防治的实验研究。方法 对BALB/c小鼠 ,经脾脏注入指数生长期的小鼠结肠腺癌细胞 (CT2 6 )悬液 0 .1ml,含细胞 1× 10 6个 ,保留脾脏。观察接种后小鼠的生存期 ,小鼠分别于接种后第 7、10、15天及自然死亡后剖腹 ,观察腹腔内肿瘤生长情况 ,留标本作病理检查和流式细胞术 (FCM )倍体检测。结果 小鼠平均自然生存时间为 (18.2± 1.8)天 ,尸解发现接种动物的肝脏表面均有灰白色转移结节形成 ,脾脏上有结节形成 ,其他脏器未见转移病灶。病理结果示肝转移瘤细胞与脾脏肿瘤细胞结构相似 ,符合低分化腺癌的特征。FCM倍体检测结果示肝转移瘤为四倍体肿瘤 ,与CT 2 6细胞倍体检测结果一致。结论 经脾脏注入CT 2 6细胞是建立结肠癌肝转移的可靠模型 ,具有潜伏期短、可重复的高转移发生率的特点 ,有较好的应用价值。
Objective To establish an animal model of liver metastasis of colon cancer for experimental study of tumor metastasis prevention. Methods In BALB/c mice, spleen was injected into the exponentially growing mouse colon adenocarcinoma cell (CT2 6) suspension (0.1 ml) containing 1×10 6 cells, and the spleen was preserved. The survival time of the mice after the inoculation was observed. The mice were sacrificed on the 7th, 10th and 15th day after the inoculation and the natural death. The growth of the intraperitoneal tumors was observed. The left specimens were examined for pathological examination and flow cytometry (FCM) ploidy. Testing. Results The average survival time of mice was (18.2±1.8) days. Autopsy findings revealed that all the livers of the vaccinated animals had gray-white metastatic nodules and nodules were formed on the spleen. No metastases were found in other organs. The pathological results showed that the hepatic metastasis cells were similar to the spleen tumor cell structure, which was consistent with the characteristics of poorly differentiated adenocarcinoma. The results of FCM ploidy showed that the liver metastases were tetraploid tumors, which was consistent with the results of CT 2 6 cell ploidy. Conclusion The injection of CT 2 6 cells into the spleen is a reliable model to establish liver metastasis of colon cancer. It has the characteristics of short latency and high repeatable incidence of metastasis and has a good application value.