论文部分内容阅读
目的检测幽门螺杆菌(Helicobacter pylori,Hp)感染慢性胃炎、消化性溃疡和胃癌患者的粪便是否存在活的Hp,以探讨Hp的传播途径。方法收集60例胃粘膜快速尿素酶试验强阳性患者的胃粘膜和新鲜粪便,进行Hp的分离培养和PCR检测。结果52份胃粘膜标本分离到Hp,阳性率86.7%;6份粪便标本分离到Hp,阳性率10.0%,其中4份来自慢性胃炎患者,2份来自消化性溃疡患者。粪便培养阳性的患者胃粘膜培养均阳性;PCR检测同一患者两种标本分离菌株的细胞毒素相关蛋白基因(CagA)和空泡毒素信号序列s1a基因(VacA s1a)一致,其中4株为CagA+和VacA s1a+,另2株VacA s1a+和尿素酶A亚单位(UreA)基因阳性。结论Hp感染者的粪便中有活的Hp存在,该菌可能通过粪-口途径传播。
Objective To detect the presence or absence of viable Hp in Helicobacter pylori (Hp) infected patients with chronic gastritis, peptic ulcer and gastric cancer, in order to investigate the route of transmission of Hp. Methods Gastric mucosa and fresh faeces were collected from 60 patients with strong positive gastric mucosal rapid urease test, Hp isolation and culture and PCR detection. Results The positive rate of Hp was 52.7% in 52 gastric mucosa samples. The positive rate of Hp in 6 samples was 10.0%. Four of them were from chronic gastritis and two from peptic ulcer patients. Gastric mucosa were positive in patients with positive stool culture. PCR was used to detect the cytotoxin-related protein gene (CagA) and the vacuolating signal sequence s1a gene (VacA s1a) of two isolates from the same patient, of which 4 were CagA + and VacA s1a +, the other two VacA s1a + and urease A subunit (UreA) gene positive. Conclusion There is live Hp in the stool of Hp infected patients, and the bacteria may spread through fecal-oral route.