急性重症肝炎患儿的病因不清。本文作者报道了细小病毒B19感染与重症肝炎的发生、临床表现、预后等有关。作者调查了1987～1997年间的45例重症肝炎患儿,有21例无明确病因。将所有患儿的血清样品保存于-20℃,抽提DNA并作PCR以检测有无细小病毒B19(以下简称“B19”)基因。作者在编码非结构蛋白基因区中设计了两套引物(Hcl-C和F-I)做巢式 The etiology of children with acute severe hepatitis is unclear. The author of this article reports that parvovirus B19 infection is associated with the development of severe hepatitis, clinical manifestations, and prognosis. The authors investigated 45 infants with severe hepatitis from 1987 to 1997, and 21 had no definite cause. Serum samples of all children were stored at -20 ° C and DNA was extracted and subjected to PCR to detect the presence or absence of parvovirus B19 (hereinafter referred to as “B19”) gene. The authors designed two sets of primers (Hcl-C and F-I) in the region encoding non-structural protein genes to make nested