Anal atresia is an innate disorder in the development of the distal hindgut in infants, which is caused by the failure of anal cloacal membrane to rupture due to the dysplasia of posterior intestine (Cuschieri, 2001).The disorder shows a prevalence of 0.03％-0.05％, of which patients with a family history account for I ％-9％ of cases (Wood and Levitt, 2018).Both environmental factors and genetic determinants contribute to anal atresia, and the latter plays a dominant role in the pathogenesis (Wijers et al., 2014).Abnormal apoptosis is an important trigger of anal atresia (Qi et al., 2000).During the embryonic development, when the cloacal membrane shows abnormal apoptosis at the early stage of anal opening, the anal membrane would not rupture, leading to anal atresia (Qi et al., 2000).Congenital anal atresia also occurs in domestic pigs (Sus scrofa) at an incidence of 0.1％-1％ in different breeds (Thaller et al., 1996), which shows striking similarities to that in human but is nonsyndromic (Cassini et al., 2005).This provides an exceptional opportunity to dissect the molecular mechanisms of this disorder.