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目的：了解肿瘤细胞与活化Ｂ淋巴细胞的融合能否提高前者的免疫原性。方法：分别取亲本Ｂ１６黑色素瘤和融合瘤细胞Ｂ１６．Ｂ主动免疫同系小鼠，观察免疫小鼠脾脏淋巴细胞（ＳＰＬ）对亲本瘤细胞体外再刺激的反应能力。结果：发现Ｂ１６．Ｂ免疫小鼠ＳＰＬ较Ｂ１６免疫小鼠ＳＰＬ有更明显的增生反应，同时前者还能分泌更高水平的细胞因子（ＩＬ２、ＩＦＮγ和ＴＮＦα）。结论：与亲本肿瘤相比，融合瘤细胞的免疫原性有明显提高
Objective: To understand whether fusion of tumor cells with activated B lymphocytes can increase the immunogenicity of the former. METHODS: Parental B16 melanoma and hybridoma cells B16 were taken. B. Actively immunized syngeneic mice and observed the ability of immune mouse spleen lymphocytes (SPL) to respond to re-stimulation of parental tumor cells in vitro. Results: Found B16. Compared with B16-immunized mice, SPL in B-immunized mice had more pronounced hyperplasia, while the former also secreted higher levels of cytokines (IL-2, IFN-gamma, and TNF-alpha). Conclusion: Compared with parental tumors, the immunogenicity of the hybridoma cells is significantly improved