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本文利用作者建立的密度梯度超速离心分离VLDL的新方法和分析等电聚焦电泳,测定了95例中国人Apo-E基因多态性和其表型分布以及血脂水平。所得表型分布趋势与国外报告一致:E_3/E_3频率最高,E_3/E_2和E_3/E_4次之,E_4/E_4,E_4/E_2和E_2/E_2最低。同对发现中国人群的E_3/E_3表型百分分布明显高于西方人群,但未发现有E_2/E_2表型。我国人群Apo-E表型分布的这种特征可能与中国人群冠心病的患病率较西方人群为低有关。血清脂质测定和分析表明,具有不同表型人群的血脂水平无显著的统计学差异。
In this paper, a new method of ultracentrifugation of VLDL by density gradient centrifugation and the analysis of isoelectric focusing were used to determine the polymorphism of Apo-E gene in 95 Chinese and its phenotype distribution and blood lipid levels. The trend of phenotypic distribution was consistent with the foreign reports: E_3 / E_3 had the highest frequency, E_3 / E_2 and E_3 / E_4 followed by E_4 / E_4, E_4 / E_2 and E_2 / E_2. In the same cohort, the percentage of E_3 / E_3 phenotypes in Chinese population was significantly higher than in Western populations, but no E_2 / E_2 phenotype was found. This characteristic of Apo-E phenotype distribution in Chinese population may be related to the lower prevalence of coronary heart disease in Chinese population than in western population. Serum lipids measurement and analysis showed that there was no statistically significant difference in the level of blood lipids between populations with different phenotypes.