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目的检测钒氧化物、电离辐射、尘肺、黄磷四种接害工人外周血中EGFR和KRAS基因突变情况。方法分别对102名接触钒氧化物、164名接触电离辐射、88名接触黄磷工人以及78名尘肺患者进行问卷调查(性别、年龄、工种和接触时间),采集外周静脉血样品并提取DNA,采用聚合酶链式反应-限制性片段长度多态性分析(PCR-RFLP)检测EGFR基因外显子21中的L858R点突变(CTG→CGG,Leu→Arg)和KRAS基因外显子2上的G12C(GGT→TGT,Gly→Cys)、G12V(GGT→GTT,Gly→Val)和G12D(GGT→GAT,Gly→Asp)等点突变情况。结果四种接害工人外周血中均未检测到EGFR基因外显子21中L858R点突变和KRAS基因第12位密码子的点突变。结论钒氧化物、电离辐射、尘肺、黄磷四组接害工人外周血DNA均未发现EGFR基因外显子21中的L858R突变和KRAS基因外显子2中第12位密码子的点突变。
Objective To detect the mutations of EGFR and KRAS gene in peripheral blood of workers exposed to vanadium oxide, ionizing radiation, pneumoconiosis and yellow phosphorus. Methods Peripheral venous blood samples were collected and DNA was extracted from 102 volunteers exposed to vanadium oxide, 164 exposed to ionizing radiation, 88 exposed yellow phosphorus workers and 78 pneumoconiosis patients (gender, age, type of work and contact time) Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect point mutations in L858R (CTG → CGG, Leu → Arg) in exon 21 of EGFR gene and in exon 2 of KRAS gene G12C (GGT → TGT, Gly → Cys), G12V (GGT → GTT, Gly → Val) and G12D (GGT → GAT, Gly → Asp). Results No point mutation of L858R in exon 21 of EGFR gene and codon 12 of KRAS gene were detected in peripheral blood of the four workers. Conclusion No mutation of L858R in exon 21 of EGFR gene and point mutation of codon 12 in exon 2 of KRAS gene were found in peripheral blood DNA of workers exposed to vanadium oxide, ionizing radiation, pneumoconiosis and yellow phosphorus.