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以小麦幼穗、幼胚为原始材料,进行耐盐突变体的筛选,并对其愈伤组织及再生植株后代进行耐盐稳定性的生理生化分析.结果表明:耐盐系在高盐浓度下其鲜重、干重明显高于对照系;耐盐系保持较高的K+/Na+比;与对照相比,耐盐系种子醇溶蛋白电泳带为14条,其中B2,B3,B4带为耐盐系所特有,B1带含量高于对照.但对照B5带合成量增加;耐盐系再生植株后代可溶蛋白电泳带耐盐系为28条蛋白带,而对照系为26条蛋白带,98kD,75kD,52kD,49kD和32kD为耐盐系所特有蛋白带,而38kD,35kD和21kD蛋白带为对照系所特有.
Salt-tolerant mutants were screened from young ears and young embryos of wheat. Physiological and biochemical analyzes of salt-tolerant stability of callus and regenerated plants were carried out. The results showed that the fresh weight and dry weight of salt-tolerant lines were significantly higher than those of the control lines at high salt concentrations, while the salt-tolerant lines maintained high K + / Na + ratios. Compared with the control, the salt-tolerant seed prolamin bands were Among them, B2, B3 and B4 belonged to salt-tolerant lines, and B1 belonged to higher content than control. However, the amount of the synthetic B5 band was increased. The salt-tolerant lines of the soluble protein electrophoresis bands of the regenerated plants were 28 protein bands, whereas the control lines consisted of 26 bands with 98kD, 75kD, 52kD, 49kD and 32kD salt tolerance Unique protein bands, whereas the 38 kD, 35 kD and 21 kD protein bands are peculiar to the control line.