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为开发利用水稻广亲和基因S5-n的功能标记筛选了部分资源以及鉴定了以培矮64S不育系为母本的杂交种种子纯度。应用广亲和基因S5-n的功能标记S5136从不育系中筛选出携带广亲和基因S5-n的不育系粤泰A,并利用测序证实,同时发现在缺失下游有1个单碱基的突变,与来源于印度的广亲和材料相同;对以培矮64S为母本的杂交稻两优986的种子中不育系自交结实情况进行了判定,从233棵苗中发现4株培矮64S,自交结实率为1.72%。同时比较了SDS方法和简易DNA快速提取方法的PCR结果,发现利用简易方法提取的DNA当天扩增也可以获得较好的PCR结果,但常温保存2周后,以简易方法提取的DNA为模板难以获得PCR产物。因此,S5-n基因的功能标记S5136可以用于广亲和基因的资源鉴定以及鉴定以培矮64S为母本的杂交种自交结实率。
Some resources were screened for the development of functional markers of rice wide-compatibility gene S5-n and the purity of hybrid seeds with Pei’ai 64S as the female parent was identified. Application of wide affinity gene S5-n function marker S5136 from male sterile lines screened a broad-compatibility gene S5-n male sterile line Yuetai A, and confirmed by sequencing, also found in the absence of a single base The mutation of the base was the same as the broad compatibility material from India. The selfing and solid state of CMS lines in hybrid rice Liangyou 986 with Pei’ai 64S as the female parent was determined. Among the 233 seedlings, 4 Plant Pei-dwarf 64S, self-fertility rate was 1.72%. At the same time, the PCR results of SDS method and simple DNA extraction method were compared. It was found that the DNA extracted by simple method could obtain better PCR results at the same time. However, DNA extracted by simple method was difficult to be obtained after 2 weeks at room temperature The PCR product was obtained. Therefore, S5136, a functional marker of S5-n gene, can be used for resource identification of broad-compatibility genes and self-fertility of hybrids with Pei’ai 64S as the female parent.