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目的评价孕期乙酸铅暴露对性成熟雄性子代小鼠睾丸组织能量代谢酶活力的影响,并探讨其对生殖系统损伤的作用机制。方法将清洁级健康昆明种孕小鼠32只按体重随机分为高剂量组(2 000mg/kg)、中剂量组(1 000mg/kg)、低剂量组(500mg/kg)和对照组(蒸馏水),每组8只。于妊娠第14d开始,每日灌胃染毒,灌胃剂量为10ml/kg,持续至母鼠自然分娩。记录孕鼠染毒期间体重增量和分娩时间。待雄性仔鼠8周龄(性成熟)时,称重并处死,分离双侧睾丸和附睾,测定睾丸、附睾的重量及脏器系数、附睾精子数。检测睾丸组织中的碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、Ca-Mg-ATP酶、Na-K-ATP酶、总ATP酶活力。结果各组孕鼠分娩时间和体重增量间比较,差异均无统计学意义(P均>0.05)。中、高剂量组仔鼠体重、睾丸重量、睾丸脏器系数、附睾重量、附睾脏器系数均明显低于对照组(P<0.05);各染毒组仔鼠附睾精子数、睾丸组织SDH酶、Na-K-ATP酶、Ca-Mg-ATP酶、总ATP酶活力均明显低于对照组(P均<0.01);高剂量组仔鼠睾丸组织LDH酶、AKP酶活力均明显低于对照组(P<0.01);且随着乙酸铅染毒剂量的增高,睾丸组织SDH酶、Na-K-ATP酶、Ca-Mg-ATP酶以及总ATP酶活力均呈下降趋势。结论在本研究的染毒时间和剂量范围内,孕期乙酸铅暴露可干扰雄性子代小鼠睾丸的能量代谢,继而使得精子生成减少。
Objective To evaluate the effects of lead exposure during pregnancy on the energy metabolism of testis in sexual maturity male mice and to explore its mechanism of action on the reproductive system injury. Methods Thirty-two healthy pregnant Kunming mice were randomly divided into high dose group (2 000 mg / kg), middle dose group (1000 mg / kg), low dose group (500 mg / kg) and control group ), 8 in each group. On the 14th day of gestation, the rats were intragastrically administered with a daily dose of 10ml / kg until the mice were given natural delivery. Record pregnant rats during exposure to weight gain and delivery time. When male offspring were 8 weeks old (sexual maturity), the testis and epididymis were separated and weighed and sacrificed. The weight of the testis and epididymis, organ coefficient and epididymal sperm count were determined. Alkaline phosphatase (AKP), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), Ca-Mg-ATPase, Na-K-ATPase and total ATPase activity in testis tissue were detected. Results There was no significant difference in the time of delivery and weight gain between pregnant rats in each group (all P> 0.05). The body weight, testicular weight, testicular organ coefficient, epididymis weight and epididymis organ coefficient were significantly lower in middle and high dose group than in control group (P <0.05). The numbers of epididymal sperm, testicular tissue SDH enzyme , The activities of Na-K-ATPase, Ca-Mg-ATPase and total ATPase were significantly lower than those of the control group (all P <0.01). The activities of LDH and AKP in testis were significantly lower than those in the control (P <0.01). The activities of SDH, Na-K-ATPase, Ca-Mg-ATPase and total ATPase in testis decreased with the increase of lead acetate dose. Conclusion Exposure to lead acetate in pregnancy during the exposure time and dose range in this study can interfere with the energy metabolism of male testes in male offspring, which in turn leads to reduced sperm production.